Lipase 01（SeeZymes（TM））

Cat No.

• SMEZ-Lip01

Description

• Lipoprotein lipase (LPL) (EC 3.1.1.34) is a member of the lipase gene family, which includes pancreatic lipase, hepatic lipase, and endothelial lipase. It is a water soluble enzyme that hydrolyzes triglycerides in lipoproteins, such as those found in chylomicrons and very low-density lipoproteins (VLDL), into two free fatty acids and one monoacylglycerol molecule. It is also involved in promoting the cellular uptake of chylomicron remnants, cholesterol-rich lipoproteins, and free fatty acids.

Source

• Microorganism

Applications

• This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α-glycerophosphate oxidase and glycerol kinase. Usually, the reaction can be completed in 5 minutes at 37°C by using 2.5~3.0 units of the enzyme per test (3.0ml) at pH around 7.0.

Appearance

• Light brown amorphous powder, lyophilized

Enzyme Commission Number

• EC 3.1.1.34

Activity

• >1.0U/mg-solid or more

CAS No.

• 9004-02-8

Contaminants

• Phosphatase 1.0×10⁻³% Catalase 2.0×10⁻²% NADH oxidase1.0×10⁻³% Cholesterol oxidase 2.0×10⁻³%

pH Stability

• pH 5.5-10.0 (25°C, 20hr)

Optimum pH

• 8

Optimum temperature

• 40-45°C

Thermal stability

• below 55°C (pH 7.0, 15min)

Stability

• Store at -20°C