Laccase

Cat No.

• SMEZ-Laa01

Description

• Laccase is a blue copper oxidase that reduces molecular oxygen to water. Laccase oxidizes polyphenols, methoxy-substituted phenols and diamines, but not tyrosine. Oxidation by laccase is an one-electron reaction that generates a free radical.

Abbr

• Laccase, Recombinant (Escherichia coli)

Species

• Escherichia coli

Form

• 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol

Enzyme Commission Number

• EC 1.10.3.-

Molecular Weight

• 55.4 kDa

Purity

• >90% as judged by SDS-PAGE

Concentration

• 1 mg/mL

Specificity

• 2,20-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)

Optimum pH

• 6.5

Optimum temperature

• 55 °C

Storage

• This enzyme is shipped at room temperature but should be stored at -20 °C.

Synonyms

• Laccases; EC 1.10.3.2; 80498-15-3; urishiol oxidase; urushiol oxidase; p-diphenol oxidase; benzenediol:oxygen oxidoreductase