Glucose Dehydrogenase

Cat No.

• SMEZ-Glc01

Description

• In enzymology, a glucose 1-dehydrogenase (EC 1.1.1.47) is an enzyme that catalyzes the chemical reaction:beta-D-glucose + NAD (P)+↔ D-glucono-1,5-lactone + NAD (P)H + H+. The 3 substrates of this enzyme are beta-D-glucose, NAD+, and NADP+, whereas its 4 products are D-glucono-1,5-lactone, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.

Abbr

• GDH, Recombiant (E. coli)

Alias

• GDH

Source

• E. coli

Applications

• This enzyme is useful for determination of glucose.

Appearance

• Lyophilized

Enzyme Commission Number

• EC 1.1.1.47

CAS No.

• 9028-53-9

pH Stability

• 5.0 - 10.0 (with 3M NaCl)

Specificity

• D-Glucose: 100 %;
  

Unit Definition

• One unit of activity is defined as the amount of GlcDH2 that forms 1 μmol of NADH per minute at 37 °C.

Storage

• Stable at -20 °C for at least one year.

Synonyms

• EC 1.1.1.47; D-glucose dehydrogenase (NAD (P)+); hexose phosphate dehydrogenase; β-D-glucose:NAD (P)+ 1-oxidoreductase; glucose 1-dehydrogenase; Glucose dehydrogenase; 9028-53-9

Specific Activity

• more than 900 U/mg protein

Reaction

• D-Glucose + NAD(P)+ ←→ D-Glucono-δ-lactone + NAD(P)H + H+